P-element


StanExPelement

“The StanEx P-element is based on the InSITE design. It contains the open reading frame (ORF) encoding a fusion of the bacterial LexA DNA binding domain to the hinge and transactivation domain of Gal4 from yeast (LexA::HG). We chose LexA::HG over the more conventional LexA::VP16 fusion because of its reported lower toxicity. The LexA::HG is followed by the 3’ untranslated region (UTR) of the heat shock protein 70 (hsp70) gene. The P-element also contains the attP and loxP sites for site specific recombinases FC31 and Cre, respectively to facilitate recombinase-mediated cassette exchange (RMCE). These sites allow us to exchange the LexA::HG cassette for a number of other genetic elements while maintaining the positional information of the original P-element” (StanEx Manual w/ Edits).